A collection of protocols covering a variety of methods to achieve super resolution in microscopy to include structured illumination, single-molecule fluorescence and localization-based approaches.

Microscopy continues to transform the life sciences. Here are five recent breakthroughs made possible by the technique.

Researchers have shown that consumer-grade 3D printers and low-cost materials can be used to produce multi-element optical ...

To unravel the complexities of biological phenomena, scientists have long relied on microscopy to visualize the intricate details of their specimens, including tissue architecture, cell morphology, ...

Researchers have demonstrated that consumer-grade 3D printers and inexpensive materials can produce advanced optical ...

Even those who maintain that super-resolution microscopy is a powerful tool of biological discovery have admitted that it may have a bit of an image problem. For example, in a recent review, several ...

Advanced light microscopy techniques are giving scientists a new understanding of human biology and what goes wrong in diseases Katarina Zimmer, Knowable Magazine Innovative techniques are helping ...

When single-molecule super-resolution microscopes were first commercialized some 15 years ago, they made headlines for their ability to resolve individual molecules and structures at the nanometer ...

Overcoming the resolution limit in a light microscope of around half a wavelength of light (about 250 nanometers) is one of the most significant developments in optics. Due to the wave nature of light ...

Top: Random Illumination Microscopy (RIM) utilizes random speckle patterns to achieve super-resolution imaging of biological tissues, improving resolution by a factor of two compared to the optical ...

Current calibration methods rely on artificially constructed DNA structures or specific cellular features, each with significant drawbacks. DNA-based rulers require complex chemical synthesis and only ...